7WZY
Crystal structure of Adenosine triphosphate phosphoribosyltransferase (HisG) from Acinetobacter baumannii at 2.975 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-26 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.698, 77.833, 97.239 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.765 - 2.975 |
| Rwork | 0.221 |
| R-free | 0.29660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wgm |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.293 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.765 | 3.260 |
| High resolution limit [Å] | 2.975 | 2.975 |
| Rmerge | 0.052 | |
| Number of reflections | 6663 | 334 |
| <I/σ(I)> | 8.6 | 1.6 |
| Completeness [%] | 90.5 | 73.2 |
| Redundancy | 4.2 | 3 |
| CC(1/2) | 0.998 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1M Sodium acetate trihydrate (pH 4.6), 2M Sodium formate |
