7WZO

Crystal structure of the SARS-CoV-2 nucleocapsid protein N-terminal domain in complex with Ubl1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97581
Spacegroup name	P 31 2 1
Unit cell lengths	69.537, 69.537, 269.253
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.020 - 2.640
R-factor	0.2161
Rwork	0.214
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vnu
RMSD bond length	0.009
RMSD bond angle	1.084
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.020	2.770
High resolution limit [Å]	2.640	2.640
Rmerge	0.150	1.187
Rpim	0.049	0.429
Number of reflections	23164	3003
<I/σ(I)>	13.2	2.4
Completeness [%]	99.7
Redundancy	18.7
CC(1/2)	0.997	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	0.1 M sodium citrate, pH 5.0, 20% w/v PEG 8000