7WZL

Crystal structure of Cytochrome P450 184A1 from streptomyces avermitilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-06-16
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	55.668, 62.071, 134.296
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.290 - 2.270
R-factor	0.2064
Rwork	0.204
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l8i
RMSD bond length	0.008
RMSD bond angle	1.550
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.310
High resolution limit [Å]	2.270	2.270
Rmerge	0.111	0.491
Number of reflections	21998	21998
<I/σ(I)>	56.434	7.883
Completeness [%]	99.2
Redundancy	13.8
CC(1/2)		0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288.15	15% PEG3350, 0.2 M sodium chloride, 0.1M HEPES, pH 7.0, 5% MPD