7WZA

An open conformation Form 1 of switch II for RhoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9875
Spacegroup name	P 21 21 21
Unit cell lengths	31.412, 67.368, 83.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.684 - 1.500
R-factor	0.188761435457
Rwork	0.187
R-free	0.21973
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ftn
RMSD bond length	0.007
RMSD bond angle	0.927
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.684	1.554
High resolution limit [Å]	1.500	1.500
Rmeas	0.128
Rpim	0.036	0.403
Number of reflections	28558	2367
<I/σ(I)>	23.15
Completeness [%]	99.8
Redundancy	12.2
CC(1/2)	0.992	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	20% PEG 3350, 0.2M potassium formate