7WZ9
HSA-In agent complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-08 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.900, 38.530, 95.620 |
| Unit cell angles | 90.00, 105.40, 90.00 |
Refinement procedure
| Resolution | 92.190 - 2.830 |
| R-factor | 0.2279 |
| Rwork | 0.223 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.699 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.880 |
| High resolution limit [Å] | 2.500 | 2.830 |
| Rmerge | 0.051 | 0.035 |
| Number of reflections | 16176 | 717 |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG3350, 50 mM potassium phosphate (pH 7.5), 5% glycerol, 4% DMSO |
