7WYR
Crystal structure of Cypovirus Polyhedra mutant fused with CLN025
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 104.520, 104.520, 104.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.707 - 1.750 |
| Rwork | 0.173 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oh6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.544 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 19331 | 489 |
| <I/σ(I)> | 5.84 | |
| Completeness [%] | 100.0 | |
| Redundancy | 211.2 | |
| CC(1/2) | 0.980 | 0.319 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 293 | in cell crystallization |
