7WXZ

Crystal structure of the recombinant protein HR121 from the S2 protein of SARS-CoV-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-12-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	65.160, 51.730, 119.160
Unit cell angles	90.00, 95.33, 90.00

Refinement procedure

Resolution	32.460 - 2.410
R-factor	0.2511
Rwork	0.249
R-free	0.29370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lxt
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.460	2.496
High resolution limit [Å]	2.410	2.410
Number of reflections	30888	30877
<I/σ(I)>	10.62
Completeness [%]	99.0
Redundancy	2
CC(1/2)	0.990	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2M sodium fluoride, 20% w/v Polyethylene glycol 3350