7WXO

Crystal Structure of PL-5 family polysaccharide lyase PanPL from Pandoraea apista at pH8.5 in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.07234
Spacegroup name	P 21 21 21
Unit cell lengths	35.922, 85.481, 90.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.250 - 1.960
R-factor	0.1978
Rwork	0.196
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wxj
RMSD bond length	0.003
RMSD bond angle	0.656
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.250	1.993
High resolution limit [Å]	1.959	1.959
Rmerge	0.102	0.810
Rpim	0.030	0.235
Number of reflections	20836	1047
<I/σ(I)>	19.2	3.3
Completeness [%]	100.0	100
Redundancy	12.8	12.6
CC(1/2)	0.998	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295.15	0.1 M Tris pH 8.5, 25% w/v Polyethylene glycol 3,350