7WXN

Crystal Structure of PL-5 family polysaccharide lyase PanPL from Pandoraea apista at pH7.5 in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-04-28
Detector	BRUKER PHOTON 100
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	36.028, 84.620, 91.301
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.060 - 2.400
R-factor	0.2471
Rwork	0.245
R-free	0.28540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wxj
RMSD bond length	0.004
RMSD bond angle	0.848
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.060	2.240
High resolution limit [Å]	2.140	2.140
Rmerge	0.185	0.488
Number of reflections	15538	1605
<I/σ(I)>	10.54	2.51
Completeness [%]	96.6	80.3
Redundancy	10.37	2.19
CC(1/2)	0.961	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295.15	0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350