7WXK

Crystal Structure of PL-5 family polysaccharide lyase PanPL from Pandoraea apista at pH4.5 in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.07234
Spacegroup name	P 21 21 21
Unit cell lengths	45.969, 65.409, 98.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.560 - 1.680
R-factor	0.2143
Rwork	0.212
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wxj
RMSD bond length	0.006
RMSD bond angle	0.853
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.560	1.704
High resolution limit [Å]	1.675	1.675
Rmerge	0.104	0.823
Rpim	0.032	0.364
Number of reflections	34187	1525
<I/σ(I)>	17.2	2.1
Completeness [%]	97.5	88
Redundancy	11.2	5.9
CC(1/2)	0.998	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	295.15	0.1 M Sodium acetate trihydrate pH 4.5, 25% w/v Polyethylene glycol 3,350