7WX0

E40K variant of Cu/Zn-superoxide dismutase from dog (Canis familiaris)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.275
Spacegroup name	P 21 21 21
Unit cell lengths	38.781, 50.677, 185.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.940 - 1.400
R-factor	0.17174
Rwork	0.171
R-free	0.19061
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.865
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.890	1.480
High resolution limit [Å]	1.400	1.400
Number of reflections	70224	8778
<I/σ(I)>	26.6
Completeness [%]	95.7
Redundancy	18.4
CC(1/2)	1.000	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 3350, 0.2M Sodium Malonate, pH 7.0