7WWN
Structure of hypothetical protein TTHA1873 from Thermus thermophilus with Potassium mercuric iodide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-06-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.570, 42.570, 156.049 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.040 - 2.050 |
| R-factor | 0.2066 |
| Rwork | 0.204 |
| R-free | 0.26310 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.656 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.020 | 2.090 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 8938 | 195 |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 89.6 | |
| Redundancy | 22.6 | |
| CC(1/2) | 0.998 | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1 M Tris pH 8.5 and 3.0 M Sodium chloride |
