7WWE

Crystal structure of Saccharomyces cerevisiae Sfh2 in an apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	110
Detector technology	CCD
Collection date	2020-08-04
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	42.920, 77.976, 53.628
Unit cell angles	90.00, 95.17, 90.00

Refinement procedure

Resolution	23.620 - 2.200
R-factor	0.2024
Rwork	0.196
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.235
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.053	0.068
Rpim	0.027	0.033
Number of reflections	17067	902
<I/σ(I)>	44.1
Completeness [%]	94.9	99.4
Redundancy	4	4.2
CC(1/2)	0.997	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M HEPES-NaOH pH 7, 0.1M KNO3, 30% PEG3350