7WW5

Crystal structure of MutT-8-oxo-dGTP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-04
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	37.870, 55.890, 59.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.710 - 1.570
R-factor	0.1872
Rwork	0.186
R-free	0.20660
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3a6t
RMSD bond length	0.006
RMSD bond angle	1.216
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.710	1.610
High resolution limit [Å]	1.570	1.570
Rmerge	0.090	0.947
Number of reflections	18198	1312
<I/σ(I)>	14.4
Completeness [%]	100.0
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	potassium sodium tartrate, sodium citrate, ammonium sulfate