7WW0

The crystal structure of FinI complex with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 2 21
Unit cell lengths	73.832, 110.062, 123.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.400 - 1.850
R-factor	0.1775
Rwork	0.176
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wvs
RMSD bond length	0.012
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.12)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.830	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.065	1.117
Rpim	0.027	0.498
Number of reflections	86691	4535
<I/σ(I)>	22	2.2
Completeness [%]	100.0	100
Redundancy	13	11.5
CC(1/2)	0.999	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M Ammonium sulfate, 0.1 M MES pH 6.5, 20%(w/v)PEG8000