7WVS

The structure of FinI complex with SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 2 21
Unit cell lengths	73.555, 108.760, 124.176
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.600 - 2.350
R-factor	0.1725
Rwork	0.170
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wup
RMSD bond length	0.007
RMSD bond angle	0.798
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX (1.12-2829)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.810	2.410
High resolution limit [Å]	2.350	2.350
Rmerge	0.199	0.640
Rmeas	0.215	0.691
Rpim	0.081	0.259
Number of reflections	42257	3082
<I/σ(I)>	8.1	2.1
Completeness [%]	99.0	100
Redundancy	13.3	13.7
CC(1/2)	0.991	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	0.2 M Lithium sulfate, 0.1 M MES pH 6, 20%(w/v)PEG4000