7WUW
Crystal structure of AziU3/U2 from Streptomyces sahachiroi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2020-12-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.851, 132.203, 157.015 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.711 - 1.750 |
| Rwork | 0.169 |
| R-free | 0.19560 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.193 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.711 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.089 | 0.795 |
| Rmeas | 0.096 | 0.861 |
| Number of reflections | 115932 | 5707 |
| <I/σ(I)> | 14.4 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.999 | 0.824 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 20% PEG 3350, 0.2M Magnesium formic acid |
