7WUN

Crystal structure of UBR bof from PRT6 (RSG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-08-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	29.803, 29.803, 114.755
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.540 - 1.530
R-factor	0.1692
Rwork	0.167
R-free	0.18610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1300027541
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.560
High resolution limit [Å]	1.530	4.150	1.530
Rmerge	0.115	0.052	0.519
Rmeas	0.123	0.056	0.578
Rpim	0.041	0.020	0.240
Total number of observations	82066
Number of reflections	9542	569	418
<I/σ(I)>	7.4
Completeness [%]	99.2	98.8	88.4
Redundancy	8.6	8	4.1
CC(1/2)		0.999	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1M sodium citrate, 200mM NaCl, 0.1M Tris