7WUM

Crystal structure of UBR box from PRT6 (RDG pH 8.5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2021-07-10
Detector	RAYONIX MX-300
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	29.756, 29.756, 115.566
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.770 - 1.580
R-factor	0.1822
Rwork	0.179
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1300027541
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.610
High resolution limit [Å]	1.580	4.290	1.580
Rmerge	0.075	0.053	0.321
Rmeas	0.082	0.058	0.361
Rpim	0.031	0.024	0.158
Number of reflections	8791	517	292
<I/σ(I)>	8.4
Completeness [%]	98.2	97.5	69.7
Redundancy	7.7	6	3.8
CC(1/2)		0.996	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	1M sodium citrate, 200mM NaCl and 0.1M Tris