7WUK
Crystal structure of UBR bof from PRT6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-07-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.2831 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 29.749, 29.749, 115.636 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.780 - 1.630 |
R-factor | 0.1881 |
Rwork | 0.186 |
R-free | 0.21850 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.833 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.690 |
High resolution limit [Å] | 1.630 | 3.510 | 1.630 |
Rmerge | 0.041 | 0.021 | 0.130 |
Rmeas | 0.044 | 0.023 | 0.143 |
Rpim | 0.018 | 0.009 | 0.058 |
Total number of observations | 88259 | ||
Number of reflections | 14336 | 1452 | 1420 |
<I/σ(I)> | 15.8 | ||
Completeness [%] | 99.9 | 99.4 | 100 |
Redundancy | 6.2 | 6.3 | 5.9 |
CC(1/2) | 1.000 | 0.992 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 8% (v/v) Tacsimate, 20% PEG 3350 |