7WT4
Crystal structure of HLA-A*2402 complexed with 8-mer Influenza PB1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-24 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.122, 46.434, 141.529 |
Unit cell angles | 90.00, 103.97, 90.00 |
Refinement procedure
Resolution | 47.708 - 1.895 |
R-factor | 0.201452304898 |
Rwork | 0.200 |
R-free | 0.23177 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f7t |
RMSD bond length | 0.008 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.010 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.078 | 0.472 |
Number of reflections | 85081 | 13627 |
<I/σ(I)> | 11.56 | |
Completeness [%] | 97.6 | |
Redundancy | 2.9 | |
CC(1/2) | 0.997 | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 100mM Tris, 20% PEG 6000 |