7WSK

Crystal structure of SARS-CoV-2 Omicron spike receptor-binding domain in complex with civet ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65 2 2
Unit cell lengths	152.665, 152.665, 187.116
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.660 - 3.300
R-factor	0.225
Rwork	0.223
R-free	0.27520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lzg
RMSD bond length	0.004
RMSD bond angle	0.627
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.453	1.549
Number of reflections	19998	1951
<I/σ(I)>	5.9
Completeness [%]	99.7
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Potassium thiocyanate, 20% w/v Polyethylene glycol 3,350