7WSJ
Crystal structure of the tandem B-box domain of Arabidopsis thaliana CONSTANS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.28176 |
| Spacegroup name | H 3 |
| Unit cell lengths | 159.090, 159.090, 55.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.930 - 2.400 |
| R-factor | 0.2055 |
| Rwork | 0.204 |
| R-free | 0.23630 |
| Structure solution method | SAD |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.930 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.111 | 0.584 |
| Number of reflections | 19958 | 2059 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 98.4 | 97.3 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 293 | 1.39 M Lithium Sulfate, 4.55% MPD, 85 mM Imidazole-HCl pH 6.5 |
