7WRX
Structure of Deinococcus radiodurans HerA-ADP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2021-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.011, 202.869, 411.852 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.400 |
| R-factor | 0.276419459316 |
| Rwork | 0.276 |
| R-free | 0.29078 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wrw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.239 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.900 | 3.460 |
| High resolution limit [Å] | 3.390 | 3.390 |
| Rmeas | 0.157 | 0.618 |
| Number of reflections | 120020 | 5712 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 97.5 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 289 | 0.2 M MgCl2, 0.1 M HEPES (pH 7.8), 14% PEG 3350 |
