7WRW
Structure of Deinococcus radiodurans HerA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2021-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 279.030, 279.030, 105.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.997 - 3.000 |
| R-factor | 0.22600367168 |
| Rwork | 0.225 |
| R-free | 0.24560 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.279 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.410 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmeas | 0.083 | 0.835 |
| Number of reflections | 94205 | 4621 |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M HEPES (pH7.5), 1.3M NaAc |
