7WRU

Crystal structure of the apo chicken glutamyl-tRNA synthetase 1 (EARS1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0094
Spacegroup name	P 1 21 1
Unit cell lengths	89.044, 41.762, 92.186
Unit cell angles	90.00, 100.20, 90.00

Refinement procedure

Resolution	45.360 - 2.500
R-factor	0.2262
Rwork	0.224
R-free	0.27030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3z
RMSD bond length	0.012
RMSD bond angle	1.133
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.360	2.540
High resolution limit [Å]	2.500	2.500
Rmeas	0.386	2.885
Number of reflections	23252	2352
<I/σ(I)>	8.11
Completeness [%]	98.3
Redundancy	6.5
CC(1/2)	0.976	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	85 mM HEPES (pH 7.5), 8.5% isopropanol, 15% glycerol, 40 mM NaF and 15% PEG 8000