7WRN

ESRP1 RNaseH-qRRM1 tandem domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	P 42 21 2
Unit cell lengths	70.365, 70.365, 114.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.940 - 1.850
R-factor	0.1954
Rwork	0.194
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vki
RMSD bond length	0.008
RMSD bond angle	1.002
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.108	1.084
Rmeas	0.111	1.106
Rpim	0.022	0.217
Number of reflections	25216	3581
<I/σ(I)>	26.9	3.7
Completeness [%]	100.0	100
Redundancy	25.4	25.6
CC(1/2)	1.000	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Potassium thiocyanate, 30% w/v Polyethylene glycol monomethyl ether 2000