7WRG

Crystal structure of full-length kinesin-3 KLP-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-04
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	56.138, 68.816, 253.216
Unit cell angles	90.00, 92.75, 90.00

Refinement procedure

Resolution	33.200 - 3.160
R-factor	0.2157
Rwork	0.213
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a1z 5b64 3fm8
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.160	3.160
Number of reflections	32692	3292
<I/σ(I)>	9.3	2.66
Completeness [%]	98.9
Redundancy	6.6
CC(1/2)	0.950	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.2M Sodium Malonate, 22% (w/v) PEG 3350