7WQ5

Crystal structure of Arabidopsis transcriptional factor WRINKLED1 with dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-01
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 2
Unit cell lengths	92.520, 132.050, 124.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.750 - 2.350
R-factor	0.2443
Rwork	0.243
R-free	0.27710
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.913
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20rc2_4400)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.430
High resolution limit [Å]	2.350	2.350
Rmeas	0.067	2.850
Number of reflections	32013	3176
<I/σ(I)>	32.27	1.47
Completeness [%]	99.5	99.81
Redundancy	26	26
CC(1/2)	1.000	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	PEG 4,000