7WP3
Crystal structure of the complex of proliferating cell nuclear antigen (PCNA) from Leishmania donovani with 1,5-Bis (4-amidinophenoxy) pentane (PNT) at 2.95 A resolution
Replaces: 5B7OReplaces: 5F7IExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 135.024, 150.664, 170.547 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.485 - 2.954 |
Rwork | 0.211 |
R-free | 0.25670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5B7O |
RMSD bond length | 0.007 |
RMSD bond angle | 1.310 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.490 | 3.030 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.490 | |
Number of reflections | 72691 | 4451 |
<I/σ(I)> | 11.6 | 1 |
Completeness [%] | 98.8 | 84.52 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20% PEG 3350, 200mM sodium malonate |