7WOJ
Crystal structure of HSA-Myr complex soaked with cisplatin for one week
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.932 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.910, 36.690, 90.010 |
| Unit cell angles | 90.00, 105.41, 90.00 |
Refinement procedure
| Resolution | 43.410 - 2.890 |
| R-factor | 0.2589 |
| Rwork | 0.258 |
| R-free | 0.28390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tdl |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.956 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.156 | 4.652 |
| Number of reflections | 12296 | 1202 |
| <I/σ(I)> | 14.8 | 0.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.4 | 7.5 |
| CC(1/2) | 0.973 | 0.254 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 26-32% PEG 3350, 50mM PB at pH 7.5 |
