7WOI

Structure of the shaft pilin Spa2 from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	90
Detector technology	PIXEL
Collection date	2021-09-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	45.645, 64.092, 441.981
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.430 - 2.730
R-factor	0.2669
Rwork	0.266
R-free	0.29330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold2
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.430	2.830
High resolution limit [Å]	2.730	2.730
Rmerge	0.173	0.938
Number of reflections	26950	1348
<I/σ(I)>	13.7	3.3
Completeness [%]	91.4	76
Redundancy	12.8	12
CC(1/2)	0.997	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2M Sodium sulfate, 0.1M Bis-Tris propane pH 7.5, 20% w/v PEG 3350