7WNS
Crystal structure of ENF peptide binding protein from Silkworm
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.477, 121.692, 253.769 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.543 - 1.799 |
| Rwork | 0.173 |
| R-free | 0.18530 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.997 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.799 | 1.800 |
| Rmerge | 0.082 | 0.928 |
| Number of reflections | 78393 | 3823 |
| <I/σ(I)> | 36.67 | 3 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 13.2 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | O.5M magnesium formate, 0.1M Bis-Tris pH 6.5 |
