7WNO

Crystallographic structure of copper amine oxidase from Arthrobacter glibiformis at pD 7.4 determined by only neutron diffraction data.

Experimental procedure

Experimental method	LAUE
Source type	SPALLATION SOURCE
Source details	J-PARC MLF BEAMLINE BL-03
Synchrotron site	JPARC MLF
Beamline	BL-03
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	2015-11-10
Detector	iBIX
Wavelength(s)	3.0-5.7
Spacegroup name	C 1 2 1
Unit cell lengths	157.548, 61.779, 92.232
Unit cell angles	90.00, 112.13, 90.00

Refinement procedure

Resolution	20.940 - 1.720
R-factor	0.168
Rwork	0.163
R-free	0.25700
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.141
Data reduction software	HKL-2000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.940	1.780
High resolution limit [Å]	1.720	1.720
Number of reflections	76306
<I/σ(I)>	4.99
Completeness [%]	87.4
Redundancy	2.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS		289	1.05-M potassium sodium (Na) tartrate in 25-mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer