7WN9

Dictyostelium discoideum Lactate dehydrogenase (DicLDHA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	80
Detector technology	CCD
Collection date	2020-11-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	I 41 2 2
Unit cell lengths	156.344, 156.344, 183.739
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.536 - 2.300
R-factor	0.1628
Rwork	0.161
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z1z
RMSD bond length	0.007
RMSD bond angle	0.755
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.290	2.290
Rmeas	0.141	0.511
Number of reflections	50412	4234
<I/σ(I)>	5.6
Completeness [%]	100.0
Redundancy	24.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	1.9M Ammonium sulfate, 90 mM sodium acetate pH4.6