7WLH

The crystal structure of African swine fever virus I215L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	142.302, 142.302, 39.319
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.600 - 2.650
R-factor	0.1986
Rwork	0.196
R-free	0.24070
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.845
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.740
High resolution limit [Å]	2.650	5.710	2.650
Rmerge	0.099	0.058	0.436
Rmeas	0.101	0.059	0.454
Rpim	0.023	0.014	0.120
Total number of observations	226540
Number of reflections	12713	1407	870
<I/σ(I)>	5.6
Completeness [%]	94.5	99.9	66.1
Redundancy	17.8	18.9	10.2
CC(1/2)		0.999	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium Acetate: HCl (pH 4.6), and 2.8 M Sodium Formate