7WKH
the grass carp IFNa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2021-07-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.617, 32.767, 56.762 |
| Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
| Resolution | 27.755 - 1.580 |
| Rwork | 0.188 |
| R-free | 0.22970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3piv |
| Data reduction software | HKL-3000 |
| Data scaling software | pointless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.290 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 28576 | 21056 |
| <I/σ(I)> | 33.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.971 | 0.971 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 20%(W/V) PEG 3350, 200 mM Ammonium citrate dibasic |
