7WJT
Crystal structure of coiled-coil region of mouse TMEM266
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-08 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.295, 90.484, 49.223 |
| Unit cell angles | 90.00, 101.85, 90.00 |
Refinement procedure
| Resolution | 37.910 - 2.300 |
| R-factor | 0.2136 |
| Rwork | 0.211 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a2a |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.762 |
| Data reduction software | HKL-2000 (714) |
| Data scaling software | HKL-2000 (714) |
| Phasing software | MOLREP (1.11.7.03) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.910 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.127 | 0.533 |
| Number of reflections | 11376 | 575 |
| <I/σ(I)> | 27.63 | 4.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 100 mM MES-NaOH (pH6.0), 2%(v/v) 2-propanol, 200 mM calcium acetate |
