7WJL

Crystal structure of S. cerevisiae Hos3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97893
Spacegroup name	P 2 3
Unit cell lengths	124.542, 124.542, 124.542
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.380 - 2.400
R-factor	0.1631
Rwork	0.161
R-free	0.20970
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	25383	1239
<I/σ(I)>	55.02
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	0.998	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.05 M HEPES 7.0, 0.1 M ammonium acetate, 0.02 M magnesium chloride, 8% PEG8000