7WJG
Streptococcus mutans BusR transcriptional factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9787 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.847, 72.944, 56.716 |
| Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
| Resolution | 26.680 - 2.420 |
| R-factor | 0.1913 |
| Rwork | 0.188 |
| R-free | 0.22650 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.916 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.680 | 2.440 |
| High resolution limit [Å] | 2.419 | 2.420 |
| Number of reflections | 24411 | 611 |
| <I/σ(I)> | 18.5 | |
| Completeness [%] | 98.8 | |
| Redundancy | 12 | 6.8 |
| CC(1/2) | 0.990 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1000mM Sodium citrate tribasic ,100mM Sodium cacodylate Hydrochloric acid PH6.5 |
