7WJ9
Crystal structure of Lactococcus lactis subsp. cremoris GH31 alpha-1,3-glucosidase, P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-03-07 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.400, 207.853, 117.899 |
| Unit cell angles | 90.00, 103.63, 90.00 |
Refinement procedure
| Resolution | 47.190 - 1.750 |
| R-factor | 0.18746 |
| Rwork | 0.186 |
| R-free | 0.21675 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wja |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.590 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.092 | 1.240 |
| Number of reflections | 529214 | 76483 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 100.0 | 97 |
| Redundancy | 6.9 | 6.9 |
| CC(1/2) | 0.999 | 0.642 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 17% PEG3350, 200 mM ammonium citrate buffer (pH 7.0), 3% xylitol |
