7WIN

Crystal structure of BAZ2B TAM domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-23
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.541870
Spacegroup name	P 1 21 1
Unit cell lengths	41.344, 53.860, 44.510
Unit cell angles	90.00, 96.78, 90.00

Refinement procedure

Resolution	34.190 - 1.950
R-factor	0.2025
Rwork	0.200
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7mwl
RMSD bond length	0.008
RMSD bond angle	1.598
Data reduction software	HKL-2000 (5.8.0267)
Data scaling software	HKL-2000 (3.27)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Rmeas	0.061
Number of reflections	13884	493
<I/σ(I)>	24.38
Completeness [%]	97.1
Redundancy	3.1
CC(1/2)	0.998	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30% w/v PEG 400, 0.2 M Sodium citrate tribasic dihydrate, and 0.1 M Tris pH 8.5