7WIM
Crystal structure of Arabidopsis thaliana FKBP43 N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-31 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.058, 203.914, 94.547 |
| Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
| Resolution | 47.000 - 2.290 |
| R-factor | 0.224 |
| Rwork | 0.223 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j2z |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.673 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.280 | 2.420 |
| High resolution limit [Å] | 2.290 | 2.300 |
| Number of reflections | 88501 | 8417 |
| <I/σ(I)> | 10.4 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.5 |
| CC(1/2) | 0.990 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2 M ammonium acetate, 0.1 M Tris (pH 8.5), 25% (w/v) Polyethylene glycol 3350 |
