7WHG
Lokiarchaeota gelsolin (2DGel) bound to two molecules of rabbit actin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-26 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.662, 72.057, 100.385 |
| Unit cell angles | 90.00, 92.08, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.250 |
| Rwork | 0.175 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hbt |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.316 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.400 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.165 | 1.189 |
| Rmeas | 0.183 | |
| Rpim | 0.077 | 0.578 |
| Number of reflections | 17387 | 1504 |
| <I/σ(I)> | 10.5 | 1.4 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 5.5 | 5.1 |
| CC(1/2) | 0.887 | 0.523 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.4 mM Loki2DGel 0.4 mM rabbit actin 0.1 M Tris-HCl, pH 7.0 0.2 M magnesium chloride hexahydrate 10% w/v polyethylene glycol 8000 1 mM CaCl2 |
