7WHF

Heimdallarchaeota gelsolin (2DGel) bound to rabbit actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-25
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	76.261, 100.353, 171.804
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.790 - 2.100
Rwork	0.165
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hbt
RMSD bond length	0.013
RMSD bond angle	1.374
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.061	0.651
Rmeas	0.067	0.714
Rpim	0.027	0.286
Number of reflections	63053	1312
<I/σ(I)>	24.7	1.7
Completeness [%]	95.3
Redundancy	5.9
CC(1/2)	0.954	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	0.4 mM Heimdallarchaeota 2DGel 0.4 mM rabbit actin 1 mM CaCl2 0.1 M HEPES pH 7.0 10% w/v polyethylene glycol 6000