7WGU
Crystal structure of metal-binding protein EfeO from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-13 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.907, 51.877, 117.379 |
| Unit cell angles | 90.00, 112.44, 90.00 |
Refinement procedure
| Resolution | 48.150 - 1.850 |
| R-factor | 0.2015 |
| Rwork | 0.199 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y4c |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.817 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.150 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.039 | 0.400 |
| Rmeas | 0.046 | 0.469 |
| Number of reflections | 65646 | 9994 |
| <I/σ(I)> | 18.5 | 2.48 |
| Completeness [%] | 97.7 | 92.8 |
| Redundancy | 3.8 | 3.4 |
| CC(1/2) | 0.999 | 0.918 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 160 mM ammonium sulfate, 80 mM sodium acetate trihydrate (pH 4.6), 20% PEG 4000, 20% glycerol 20% ethylene glycol |
