7WGM
Crystal structure of Adenosine triphosphate phosphoribosyltransferase (HisG) from Acinetobacter baumannii at 3.15 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.865, 79.645, 98.881 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.027 - 3.150 |
| Rwork | 0.215 |
| R-free | 0.27090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m8h |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.533 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.030 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.137 | |
| Number of reflections | 10564 | 513 |
| <I/σ(I)> | 15.8 | 2.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 15.1 | 15 |
| CC(1/2) | 0.999 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1M Sodium acetate trihydrate (pH 4.6), 2M Sodium formate |
