7WGK
Crystal structure of Adenosine triphosphate phosphoribosyltransferase (HisG) from Acinetobacter baumannii at 3.13 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.401, 80.950, 99.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.488 - 3.130 |
| Rwork | 0.219 |
| R-free | 0.29870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m8h |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.573 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.490 | 3.180 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Rmerge | 0.109 | 1.249 |
| Number of reflections | 11022 | 532 |
| <I/σ(I)> | 19.4 | 2.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 14.9 | 15 |
| CC(1/2) | 0.999 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1M Sodium Acetate Trihydrate (pH 4.6), 2M Sodium Formate |
