7WF9
Crystal structure of mouse SNX25 RGS domain in space group P41212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 70.877, 70.877, 148.539 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.550 |
| R-factor | 0.2248 |
| Rwork | 0.223 |
| R-free | 0.26360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wf8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.473 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 74.270 | 74.270 | 2.660 |
| High resolution limit [Å] | 2.550 | 8.820 | 2.550 |
| Rmerge | 0.058 | 0.048 | 0.700 |
| Rmeas | 0.062 | 0.051 | 0.752 |
| Rpim | 0.022 | 0.019 | 0.267 |
| Total number of observations | 101520 | 2465 | 12539 |
| Number of reflections | 12971 | 382 | 1541 |
| <I/σ(I)> | 13.7 | 23.3 | 2.5 |
| Completeness [%] | 99.5 | 97.6 | 99.5 |
| Redundancy | 7.8 | 6.5 | 8.1 |
| CC(1/2) | 0.998 | 0.998 | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.15M DL-Malic acid pH 7.0, 20%(w/v) Polyethylene glycol 3350 |
