7WEK

Crystal structure of the mouse Wdr47 NTD in complex with the WBR motif form Camsap3.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	58.077, 105.318, 155.972
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.180 - 3.210
R-factor	0.2754
Rwork	0.271
R-free	0.31350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wej
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	16169	1569
<I/σ(I)>	18.3	1.148
Completeness [%]	99.8
Redundancy	6.7
CC(1/2)	0.994	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.1M Tris-HCl, 20% (w/v) PEG 3350